10x Genomics

The Columbia Stem Cell Initiative Flow Cytometry core, in collaboration with the Columbia Genome Center Single Cell Analysis Core (SCC) is pleased to offer access to 10x Genomics single-cell technology.

After obtaining reagents from the Single Cell Analysis core, fully-trained users have independent access to the 10x Genomics Chromium Controller, workspace, and associated equipment for the generation of barcoded cDNA from GEM-captured cells. Barcoded cDNA can then be taken to the SCC for library preparation or sequencing. Users also have the option to perform library preparation and arrange for sequencing independently.

Key Points:

• CSCI Flow Cytometry supports only the GEM-capture and cDNA barcoding steps. Library preparation and sequencing must be arranged with the SCC or independently by researchers.
• Users can obtain 10x Next GEM reagents from the SCC when using the core's library preparation and sequencing services.
• 10x Genomics access is offered on a self-use basis only. The service is recommended for researchers who require off-hours access to the Chromium Controller and plan to use the technology regularly. Researchers whose needs are infrequent are encouraged to utilize the SCC's full-service option.
• Users must complete comprehensive training with CSCI Flow Cytometry staff before being granted access to the Chromium Control and workspace.

Please see our CSCI Flow Cytometry 10x Genomics Service document for more details.